Yes, the difference is very real and noticeable from every tuner.
I've heard the Bama Tunes are the worst mail tunes in the refinement and drive ability category, but they won't ruin your car or anything. I here good things about AED and Lund, and for longevity and safety I've heard Steeda is the best.
The dyno tune will be the best and will give you more horses than any e mail tuner can.
shaun from AED told me my steeda tunes numbers were all over the place and it wasn't safe for my car. So now I have an AED tune and it runs like champ.